The structure of the finitely generated nilpotent groups with infinite cyclic Frattini subgroups are completely determined. More exactly, the following theorem is proved. Let G be a finitely generated nilpotent group. Then the Frattini subgroup of G is infinite cyclic if and only if G has a decomposition G = S×F×T, where F is a free abelian group of rank s, T = Zm1 ⊕ Zm2 ⊕ … ⊕ Zmu, m1, m2, …, mu are square free integers greater than 1, m1|m2|… |mu,
where d1, d2, …, dr are integers and d1|d2|… |dr. Moreover, (d1, d2, …, dr; s; m1, m2, …, mu) is an isomorphic invariant of G. That is to say, if H is also a finitely generated nilpotent group with infinite cyclic Frattini subgroup, then G is isomorphic to H if and only if they have the same invariants.